Conference Proceedings
Studies on soluble ectodomain proteins of relaxin (LGR7) and insulin 3 (LGR8) receptors
Y Yan, J Cai, P Fu, S Layfield, T Ferraro, J Kumagai, S Sudo, JG Tang, E Giannakis, GW Tregear, JD Wade, RAD Bathgate
Annals of the New York Academy of Sciences | NEW YORK ACAD SCIENCES | Published : 2005
Abstract
The ectodomains of hoth the relaxin (LGR7) and the INSL3 (LGR8) receptors can be expressed on the cell surface using only a single transmembrane domain. These membrane-anchored proteins retain the ability to bind relaxin and can be cleaved from the cell surface. The subsequent LGR7 protein, 7BP, binds relaxin and can act as a functional relaxin antagonist. By contrast, the equivalent LGR8 protein 8BP does not hind relaxin or antagonize LGR8 activity. The 7BP protein has been successfully immobilized onto chemically derivatized surfaces for the capture of relaxin peptides and subsequent identification via SELDI-MS analysis. © 2005 New York Academy of Sciences.